The use of relative expression ratios from microarray hybridization experiments hinders comparison of different experimental conditions and does not indicate absolute expression levels. In the May 28
Their approach incorporates comparison with a calibrated oligonucleotide reference sample containing sequences complementary to every microarray spot. They also used spot intensity data from multiple scans at different detection sensitivities and applied linear regression algorithms to generate a common linear scale. Dudley
This method should allow for improved data comparability and estimation of true transcript abundance.