In the article "Gene analyses--sunny side up! Chemiluminescent detection systems for reporter gene assays" (D. Wilkinson, The Scientist, 13:18-20, March 29,1999), the explanation for how CoA transforms light production by firefly luciferase from a flash to a continuous glow-type pattern is incorrect. CoA is thought to work by binding to firefly luciferase and causing stimulatory conformational changes that speed up the enzyme's ability to release the product oxyluciferin.1
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