G protein-coupled receptors (GPCRs) are targets for about 35 percent of approved drugs, but a large proportion of GPCRs remain structurally uncharacterized. Producing stable and functional receptor proteins for assaying or structural assessment remains a roadblock. Teams can spend weeks iterating detergent and construct conditions before obtaining quality material. Cell-free protein synthesis (CFPS) offers a powerful alternative, using a detergent-free “stabilize as you synthesize” approach where nascent GPCRs insert spontaneously into a native-like lipid bilayer.
Download this application note to learn more about CFPS—a workflow that compresses iterative membrane protein optimization cycles from months into days.
