The CUTANA™ CUT&RUN Kit enables streamlined chromatin profiling of histone post-translational modifications (PTMs) and chromatin associated proteins.
- User-friendly: The kit includes all reagents needed to go from starting cells to isolated DNA in a robust and scalable multi-channel workflow.
- Integrated workflow solution: Part of our comprehensive CUTANA™ ecosystem, this kit pairs seamlessly with the CUTANA™ CUT&RUN Library Prep Kit (14-1001) and CUTANA™ Cloud to accelerate your path from cells to data insights.
- Ideal for diverse applications: Kit protocol is optimized for use with a variety of chromatin targets, starting cell numbers, and sample preparation methods.
- Robust controls for reliability: Included control antibodies and defined spike-ins ensure results you can trust.
- Ready to run, fully supported: Every kit comes with a manual packed with detailed protocols, quality checks, FAQs, and expert tips, plus a bench-proof Quick-Start Card and access to the Tech Support Center for additional guidance.
- Stringent quality control: Each new lot undergoes rigorous quality control checks to ensure consistent results in your research.
In CUT&RUN, pAG-MNase selectively releases antibody-bound chromatin into solution, where it is easily separated from bead-bound cells and purified to create high quality libraries for next-generation sequencing. This results in high sensitivity chromatin mapping using small numbers of starting cells and low sequencing depth (5-10 million total reads). The CUTANA™ CUT&RUN Kit is optimized for use with a variety of targets including histone PTMs, transcription factors, chromatin remodelers, epigenetic enzymes, and reader proteins. The kit is compatible with diverse samples including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 5,000 cells.
The CUT&RUN Kit Version 6 (v6) now includes two new Wash Buffer Enhancers to improve sample handling, as well as CUTANA™ DNA Purification Beads with an updated bead-to-sample ratio to preserve precise size selection while improving capture of smaller transcription factor-associated fragments. Each kit is equipped with a comprehensive selection of controls to ensure reliable results: positive (H3K4me3 and H3K27me3) and negative (IgG) control antibodies pair with SNAP-CUTANA™ spike-ins to support assay optimization and continuous monitoring, while E. coli Spike-in DNA enables data normalization. The kit utilizes multi-channel pipetting with provided 8-strip tubes to ensure seamless handling while maximizing throughput and reproducibility. CUTANA™ CUT&RUN Kits are bench-tested, scientist-approved, providing users with quality reagents for precision mapping.
| Specification | Value |
| Number of assays | Available in 24 or 48 reaction kits. Inquire for 96 reaction automation compatible kits or custom bulk orders. |
| Starting material | 500,000 down to 5,000 fresh or cryopreserved native cells or nuclei |
| Compatible chromatin targets | Broad compatibility including modified histones and chromatin-associated proteins (e.g. transcription factors) |
| Included positive controls | Rabbit anti-H3K4me3 and H3K27me3 antibodies |
| Included negative controls | Rabbit IgG Antibody |
| Included spike-in controls | SNAP-CUTANA™ K-MetStat Panel, CUTANA™ E. coli Spike-in DNA |
| Quality Control | Lot specific testing in CUT&RUN using included controls |
| Storage | Various components stored at room temperature, 4°C and -20°C |
| Stability | Stable for 1 year upon date of receipt |
| Product use | Research use only |
