CRISPR Antidotes Galore

Anti-CRISPR proteins are prevalent in phage genomes and bacterial mobile genetic elements, researchers show.

Jun 13, 2016
Anna Azvolinsky

FLICKR, NATURALISMUS Diverse groups of bacterial species encode genes that block the activity of specific CRISPR/Cas systems, according to a team from the University of Toronto and the University of Otago, New Zealand. Scanning prophages (genomes of bacteriophages integrated within bacterial genomes), Toronto’s Alan Davidson and colleagues have identified five new protein-coding anti-CRISPR genes, adding to the list of nine his group had previously identified. The team’s latest work, published today (June 13) in Nature Microbiology, highlights a way to learn how CRISPR systems work as well as a potential add-on tool for CRISPR-based gene editing.

“The authors report a beautiful application of the Red Queen theory, which posits that there is a kind of an arms race between all living organisms, and between the host and parasite in particular,” said Didier Raoult, a microbiologist at Aix-Marseille University in France who was not involved in the work. These proteins “are the phages’ answers to bacterial CRISPR systems which are a form of aggression by the bacteria,” he added.

“The discovery of anti-CRISPR proteins is not surprising in the sense that phage must develop these as part of the nature of the host-parasite co-evolution,” said Eugene Koonin of the US National Center for Biotechnology Information and the National Library of Medicine. 

Davidson’s team in 2013 found that a single prophage within the Pseudomonas aeruginosa genome conferred resistance to a host of other types of phages. So the researchers tweaked the P. aeruginosa CRISPR systems to try to understand how the prophage might affect the bacterium’s susceptibility to phage infection. They found that P. aeruginosa’s type I-F CRISPR system only prevented infection by certain types of phage. Searching within the phage genomes, the researchers found five unique genes, each with anti-CRISPR activity. In a subsequent study, the researchers identified another four genes the showed activity against type I-E CRISPR in P. aeruginosa.

For the present study, Davidson’s team broadened its search to the genomes of species within the diverse Proteobacteria phylum. Because the nine previously identified proteins had no sequence motifs in common, the researchers searched for sequences with homology to a putative transcriptional regulator gene they found adjacent to all known anti-CRISPR loci.

Davidson and colleagues then tested whether each putative anti-CRISPR gene, expressed on a plasmid, could support the ability of phage to replicate within bacteria with either a type I-E and I-F CRISPR system targeting the phage. The group identified four more anti-CRISPR genes targeting the I-F system, plus one that is able to block the activity of both type I-F and I-E CRISPR systems.

“That there are a lot of different anti-CRISPR gene families emphasizes that these genes are advantageous to a phage and that the CRISPR system poses an important barrier to phage replication,” Davidson told The Scientist. All three of the anti-CRISPR proteins the team previously tested in vitro bound and blocked CRISPR function in a distinct way.

According to Raoult, these anti-CRISPR proteins may provide a clue as to why some bacterial and archaeal species retain foreign DNA elements (such as prophages and other mobile genetic elements) that encode for resistance genes, while others do not. “This is an important question,” said Raoult. “I suspect that one difference may be these anti-CRISPR proteins.”

Anti-CRISPR proteins may also be useful experimental tools. “It’s not difficult to imagine that these proteins could be used to modulate the activity of CRISPR systems for genomic editing and engineering in the same way that Cas9 mutants are now being used, or as a way to transiently regulate the expression of CRISPR,” said Koonin.

The Toronto-led team is now working on identifying more genes against other CRISPR systems, including the widely used CRISPR/Cas9.

“This is probably just the tip of the proverbial iceberg of the total number of anti-CRISPR proteins,” Koonin told The Scientist.   

A. Pawluk et al., “Inactivation of CRISPR-Cas systems by anti-CRISPR proteins in diverse bacterial species,” Nature Microbiology, doi:10.1038/nmicrobiol.2016.85, 2016.

January 2019

Cannabis on Board

Research suggests ill effects of cannabinoids in the womb


Sponsored Product Updates

Cell culture made easy with new 24-channel pipetting heads for VIAFLO 96/384
Cell culture made easy with new 24-channel pipetting heads for VIAFLO 96/384
INTEGRA has responded to customers’ requests and developed two new 24-channel pipetting heads, extending the capabilities of its popular VIAFLO 96/384.
Horizon Discovery extends CRISPR Screening Service to primary human T cells
Horizon Discovery extends CRISPR Screening Service to primary human T cells
Horizon Discovery Group plc (LSE: HZD) (“Horizon” or “the Company”), a global leader in gene editing and gene modulation technologies, today announced the extension of its CRISPR Screening Service to includeex vivoT lymphocytes. The service extension meets the requirements of immunology-based research in drug discovery,enabling new gene targets to be identified in biologically and potentially therapeutically relevant settings.
pIC50: The Advantages of Thinking Logarithmically
pIC50: The Advantages of Thinking Logarithmically
Watch this webinar from Collaborative Drug Discovery to learn about how using pIC50 helps you get a better sense of the relative potencies, calculate the correct mean of multiple values, and select better sampling doses.
WIN a VIAFLO 96/384 to supercharge your microplate pipetting!
WIN a VIAFLO 96/384 to supercharge your microplate pipetting!
INTEGRA Biosciences is offering labs the chance to win a VIAFLO 96/384 pipette. Designed to simplify plate replication, plate reformatting or reservoir-to-plate transfers, the VIAFLO 96/384 allows labs without the space or budget for an expensive pipetting robot to increase the speed and throughput of routine tasks.