Menu

New Stem Cell Identified

Researchers isolate an easy-to-manipulate, stable, and spatially distinct pluripotent cell type.  

May 6, 2015
Anna Azvolinsky

RsPSCs (green) integrating into a nonviable mouse embryo in cultureSALK INSTITUTE FOR BIOLOGICAL STUDIES; JUN WU, DAIJI OKAMURA

Scientists have isolated and defined a new type of pluripotent cell from early mouse embryos and from monkey and human stem cell lines. The monkey- and human-derived versions of these pluripotent cells can divide and generate the three germ layers in a developing mouse embryo, providing the first demonstration that human pluripotent cells can begin a differentiation program inside mice. In their May 6 Nature paper reporting these results, developmental biologist Juan Carlos Izpisua Belmonte of the Salk Institute for Biological Studies in La Jolla, California, and his colleagues suggested that these newly identified cells may be useful for modeling early human development and might in the future be used to generate tissues and organs for clinical applications.

“I found the paper fascinating,” said George Daley, a stem cell biologist at Children’s Hospital Boston and Harvard Medical School who was not involved in the work. “It highlights a new type of pluripotent stem cell, which is among the most exciting aspects of stem cell biology over the last several years.”

The findings, said Paul Knoepfler, a stem cell biologist at the University of California, Davis, who was not involved in the work, “fit nicely into a broader concept that there are going to be ‘intermediate state’ stem cells that don’t fit so easily into binary, black-and-white ways of classifying [pluripotent cells].”

These so-called novel region-selective pluripotent stem cells (rsPSCs) are further differentiated compared to previously isolated stem cells and have more favorable characteristics for laboratory manipulation, including high cloning efficiency, stable passage in culture, and ease of genetic engineering. “All of these are advantages for applications in research and, potentially, scaling up for clinical applications,” said Daley.

RsPSCs are distinct from two other pluripotent mouse cell types that researchers have been able to stably propagate in culture—naive embryonic stem cells (ESCs), from the pre-implantation embryo, and primed epiblast stem cells (EpiSCs), derived from the post-implantation embryo.

“We believe that [rsPSCs] should have the ability to differentiate more efficiently into somatic cells . . . because they are right at the junction of pluripotency and differentiation,” said study coauthor Jun Wu, a research associate in the Belmonte lab.

In the post-implantation mouse embryo, cells are already distinguished into anterior, posterior, distal, and proximal functional orientations. The researchers sought to determine whether they could capture a cell type from the post-implantation embryo with distinct properties conferred by one of these spatial orientations. The team focused on isolating and culturing posterior cells where gastrulation occurs in the early embryo.

Wu and his colleagues found that a combination of serum-free media plus fibroblast growth factor 2 (FGF2) and Wnt signaling inhibitors resulted in stable rsPSCs.

The transcriptomes of these cells resembled those of the posterior cells of the early mouse embryo, and grafting these cells into 7.5-day-old mouse embryos resulted in efficient incorporation in the posterior, but not the other parts of the embryo. After 36 hours of culturing these chimaeric embryos, the rsPSCs proliferated and could differentiate into the developing three germ layers. The results of this 36-hour embryo incubation assay suggest that rsPSCs have the potential to fulfill their developmental program in vivo, said Jun.

A distinguishing feature of naive ESCs compared with primed EpiSCs is their ability to efficiently form colonies from single cells—a trait called high clonagenicity. Yet even though rsPSCs resemble cells from post-implantation embryos, just like the EpiSCs, these cells readily formed clones. RsPSCs also produced smaller teratomas—tumors that resemble embryos, including tissues from all three germ layers—when injected into mice. And while conventional human ESCs could not, human rsPSCs could also be grafted into the mouse embryo and form all three germ layers.

“The high clonal efficiency was surprising, as was the data that these cells form smaller teratoma,” said Knoepfler. “Anything that forms a smaller teratoma would have a potentially better safety profile for clinical applications,” he added.

The authors notes that human and nonhuman primate ESC lines could be used to create rsPSCs with similar characteristics to the mouse cells, including improved cloning efficiency.

Because of the ethical limitations of working with human embryos, the standard functional test for pluripotency of human stem cells is the mouse teratoma assay. “We are excited that this is the first demonstration that [human stem cells] can enter into the early developmental program of another species,” said Jun.

Whether human rsPSCs can generate more complicated tissue structures within mice or other animals requires further study, Belmonte told The Scientist via e-mail. “Of course,” he noted, “the ethical implications behind creating a human-animal chimera for the purpose of obtaining human tissues and organs to save lives of millions needs to be carefully evaluated.”  

Daley pointed out that this study is not the first to describe a novel pluripotency state. But these studies have been the subject of some controversy because the cells have been unstable in culture, and independent laboratories have struggled to reproduce the reported results.

“It remains to be seen whether these conditions for rsPSCs are reproducible, [but] it looks like [the authors] were very effective in isolating these cells,” he said. “The fact that this works across the spectrum of mammals suggests that it is a pretty stable state.”

That changing culture conditions can generate different kinds of stem cells is not surprising, said Knoepfler, but it is exciting. “I expect that more new cell types will be found in the future,” he said.

J. Wu et al., “An alternative pluripotent state confers interspecies chimaeric competency,” Nature, doi:10.1038/nature14413, 2015. 

Clarification (May 6): This article has been updated to clarify: Daley pointed out that this study is not the first to describe a novel pluripotency state.

February 2019

Big Storms Brewing

Can forests weather more major hurricanes?

Marketplace

Sponsored Product Updates

Bio-Rad Releases First FDA-Cleared Digital PCR System and Test for Monitoring Chronic Myeloid Leukemia Treatment Response
Bio-Rad Releases First FDA-Cleared Digital PCR System and Test for Monitoring Chronic Myeloid Leukemia Treatment Response
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), a global leader of life science research and clinical diagnostic products, today announced that its QXDx AutoDG ddPCR System, which uses Bio-Rad’s Droplet Digital PCR technology, and the QXDx BCR-ABL %IS Kit are the industry’s first digital PCR products to receive U.S. Food and Drug Administration (FDA) clearance. Used together, Bio-Rad’s system and kit can precisely and reproducibly monitor molecular response to treatment in patients with chronic myeloid leukemia (CML).
Bio-Rad Showcases New Automation Features of its ZE5 Cell Analyzer at SLAS 2019
Bio-Rad Showcases New Automation Features of its ZE5 Cell Analyzer at SLAS 2019
Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb) today showcases new automation features of its ZE5 Cell Analyzer during the Society for Laboratory Automation and Screening 2019 International Conference and Exhibition (SLAS) in Washington, D.C., February 2–6. These capabilities enable the ZE5 to be used for high-throughput flow cytometry in biomarker discovery and phenotypic screening.
Andrew Alliance and Sartorius Collaborate to Provide Software-Connected Pipettes for Life Science Research
Andrew Alliance and Sartorius Collaborate to Provide Software-Connected Pipettes for Life Science Research
Researchers to benefit from an innovative software-connected pipetting system, bringing improved reproducibility and traceability of experiments to life-science laboratories.
Corning Life Sciences to Feature 3D Cell Culture Technologies at SLAS 2019
Corning Life Sciences to Feature 3D Cell Culture Technologies at SLAS 2019
Corning Incorporated (NYSE: GLW) will showcase advanced 3D cell culture technologies and workflow solutions for spheroids, organoids, tissue models, and applications including ADME/toxicology at the Society for Laboratory Automation and Screening (SLAS) conference, Feb. 2-6 in Washington, D.C.