Technologies Vie for Dominance

Courtesy of Bio-Rad Laboratories  AUTOMATING DISCOVERY: Two-dimensional gel-based proteomics is traditionally derided for its technical difficulty, low-throughput, and lack of reproducibility. Instrument manufacturers have fired back with a range of automated and integrated options. Shown here is the ProteomeWorks product line. Thierry Rabilloud has been doing proteomics since long before the word proteome was even coined. For years Rabilloud, currently at the Atomic Energy Commission Re

Jeffrey Perkel
Mar 9, 2003
Courtesy of Bio-Rad Laboratories
 AUTOMATING DISCOVERY: Two-dimensional gel-based proteomics is traditionally derided for its technical difficulty, low-throughput, and lack of reproducibility. Instrument manufacturers have fired back with a range of automated and integrated options. Shown here is the ProteomeWorks product line.

Thierry Rabilloud has been doing proteomics since long before the word proteome was even coined. For years Rabilloud, currently at the Atomic Energy Commission Research Center in Grenoble, France, used two-dimensional gel electrophoresis (2-DE) to break complex protein mixtures down into their component parts, purified individual proteins that interested him, and then sent these out for identification by mass spectrometry (MS). But at this point his research hit a bottleneck: the MS facility could accept only a few samples at a time, because the sample processing procedure was so labor-intensive. Then the MS lab acquired robots to automate the processing steps, and the bottleneck disappeared. "Before we were sending...