M. Serrano, A.W. Lin, M.E. McCurrach, D. Beach, S.W. Lowe, "Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a," Cell, 88:593-602, March 7, 1997. (Cited in more than 180 papers since publication)
Comments by Scott W. Lowe, associate professor, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Scott W. Lowe and postdoctoral fellow Maria Soengas
An unpublished 15-year-old observation of an obscure cell line, dusted off and revisited, helped Scott W. Lowe and colleagues better understand oncogene cooperation. During Lowe's grad student years in the mid-'80s, he worked with Massachusetts Institute of Technology's Earl Ruley
(now a professor at Vanderbilt University). Ruley had previously inferred that the oncogene ras
might cause cell cycle arrest in the "strange" cell line REF52.1
REF52 "was unlike most immortal cell lines in that ras
would require two oncogenes to transform it, whereas in the classic 3T3 line,...