I. Bentwich et al., "Identification of hundreds of conserved and nonconserved human microRNAs," Nat Gen , 37:766-70, 2005. (Cited in 148 papers) [PUBMED]
Isaac Bentwich and colleagues at Rosetta Genomics upped the number of sequenced human microRNAs using a new technique integrating bioinformatic predictions with microarray analysis, cloning, and sequencing. Of the 89 new human microRNAs sequenced, 53 are unique to primates, lending credence to the suggestion that the short segments may be what Bentwich calls, "the switches that drive evolution."
The new tool:
Bentwich says that using computers to comb genomes for microRNAs was a relatively new approach when his group began the project in 2000. "Biologists were used to using lab coats and plastic tubes as their mainstays for finding genes," he says.
"Our work sort of broke the sound barrier of a previous notion...
|Human microRNAs identified|
|Before publication of the paper: 222|
|Upon publication of the paper: 331|
|As of July 2007: 475|