MOLECULAR BIOLOGY

Michel Philippe (Universite de Rennes, Rennes Cédex, France): "In yeast, two critical points of the cell cycle (Start and G1/S) are regulated by the same protein. This protein, called p34cdc2, is coded by the genes cdc2 in Schizzosaccharomyces pombe and CDC28 in Saccharomyces cerevisiae. By complementation of yeast mutants, proteins from higher eucaryotes homologous to cdc2 have been cloned. Moreover, p34cdc2 has been shown to be one of the main components of the well-known M-phase promotin

Paris
Jul 19, 1992

Michel Philippe (Universite de Rennes, Rennes Cédex, France): "In yeast, two critical points of the cell cycle (Start and G1/S) are regulated by the same protein. This protein, called p34cdc2, is coded by the genes cdc2 in Schizzosaccharomyces pombe and CDC28 in Saccharomyces cerevisiae. By complementation of yeast mutants, proteins from higher eucaryotes homologous to cdc2 have been cloned. Moreover, p34cdc2 has been shown to be one of the main components of the well-known M-phase promoting factor. Thus, it seemed very likely that p34cdc2 in all eucaryotes was acting at both the G1/S and G2/M transitions.

"In our lab, several clones have been selected by differential screening using the early development of the frog, Xenopus laevis, as a model system. One of them, called CDK2 (originally called Eg1), turned out to be very close to cdc2 but was unable to complement the yeast mutants. Based on the Xenopus...

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