Molecular Biology

M.C. Schmidt, C.C. Kao, R. Pei, A.J. Berk, "Yeast TATA-box transcription factor gene," Proceedings of the National Academy of Sciences, 86, 7785-9, October 1989. Arnold Berk (University of California, Los Angeles): "TFIID binding to a TATA box initiates the ordered assembly of multiple transcription factors (TFs) required for initiation on eukaryotic promoters. However, TFIID resisted purification from higher organisms, preventing detailed studies of the process. We were interested in TFIID be

The Scientist Staff
Dec 9, 1990

M.C. Schmidt, C.C. Kao, R. Pei, A.J. Berk, "Yeast TATA-box transcription factor gene," Proceedings of the National Academy of Sciences, 86, 7785-9, October 1989.

Arnold Berk (University of California, Los Angeles): "TFIID binding to a TATA box initiates the ordered assembly of multiple transcription factors (TFs) required for initiation on eukaryotic promoters. However, TFIID resisted purification from higher organisms, preventing detailed studies of the process. We were interested in TFIID because our work implied it was a target for adenovirus E1A transactivation. The breakthrough in this area came in 1988 with reports from Leonard Guarente and Phillip A. Sharp (Massachusetts Institute of Technology) (S. Buratowski, et al., Nature, 334:37-42, 1988), and Pierre Chambon (Institute of Biological Chemistry, Strasbourg, France) (B. Cavallini, et al., Nature, 334:77-80, 1988) that a protein from yeast could replace human TFIID for in vitro transcription with all other TFs and RNA polymerase from human cells. Based...