Atomic Force Microscopy

http://www.the-scientist.com/article/flash/23821/1/ Click to view enlarged diagram Credit: ILLUSTRATION: ANDREW MEEHAN" />http://www.the-scientist.com/article/flash/23821/1/ Click to view enlarged diagram Credit: ILLUSTRATION: ANDREW MEEHAN Invented in 1986 atomic force microscopy (AFM) has become a valuable tool for life scientists, offering the ability to image aqueous biological samples, like membranes, at nanometer resolution. The technique is akin to

Jul 1, 2006
Jeffrey M. Perkel
<figcaption>http://www.the-scientist.com/article/flash/23821/1/ Click to view enlarged diagram Credit: ILLUSTRATION: ANDREW MEEHAN</figcaption>
http://www.the-scientist.com/article/flash/23821/1/ Click to view enlarged diagram Credit: ILLUSTRATION: ANDREW MEEHAN

Invented in 1986 atomic force microscopy (AFM) has become a valuable tool for life scientists, offering the ability to image aqueous biological samples, like membranes, at nanometer resolution.

The technique is akin to reading Braille. Just as the blind read text by scanning their fingers across lines of text, AFM scans a material's topography using a tiny cantilever - a flexible projection, like a diving board, with a sharp, nanometer-sized probe tip protruding from the end.

Other variations on the technique are also possible: The technique can be used as a biophysical tool by measuring how much force is required to separate a ligand from a receptor. Alternatively, the AFM cantilever can be used as a pen to print biomolecular "nano-arrays," an application that at least two companies have commercialized.