TriLink’s HPLC-purified guide RNAs (gRNAs) provide a high-quality starting point for CRISPR gene editing research. While guide sequence and design are critical to editing performance, the quality of the synthesized gRNA can also influence experimental outcomes. Synthesis impurities may impact on-target activity, off-target effects, and confidence in gene editing results.
HPLC purification helps remove unwanted synthesis-related species, providing high-purity gRNAs for more confident gene editing experiments. TriLink combines this rigorous purification with advanced modification options and extended gRNA lengths, enabling researchers to explore more sophisticated editing strategies and tailor gRNAs to their specific experimental needs.
With decades of expertise in nucleic acid chemistry and manufacturing, TriLink supports custom gRNA synthesis for a broad range of CRISPR applications, from early-stage research and screening through advanced gene editing and therapeutic development.
Key features include:
- HPLC purification for high-purity gRNA
- Advanced chemical modification options
- Extended gRNA lengths for specialized editing strategies
- Custom synthesis to support diverse CRISPR workflows
- Nucleic acid expertise backed by TriLink’s experience in RNA chemistry and manufacturing
Whether you are optimizing a CRISPR workflow, evaluating a new editing strategy, or advancing gene editing research toward therapeutic applications, TriLink’s HPLC-purified gRNAs provide the quality and customization options to help you take your research further.
Product Specifications:
| Sepcification | Value |
| Purification Method | HPLC (reverse-phase standard; anion-exchange/ion-pairing available) |
| Guide Length | Up to 180 nt |
| Yield Range | 3 nmol to >100 nmol |
| Available Modifications | 2′-O-methyl, phosphorothioate (PS), 2′-fluoro, LNA, 2′-O-methoxyethyl (MOE) |
| Compatible Editing Systems | Cas9, Cas12a, base editing, prime editing, CRISPRa/i |
| Standard QC/Release | ESI-MS, PAGE |
| Optional Add-On Testing | Analytical HPLC, endotoxin, bioburden, gel densitometry, concentration |
| Formulation Options | Lyophilized (dry) or resuspended in nuclease-free water/buffer |
| Typical Turnaround | ~15 business days |
| Product Use | Research Use Only |
