Menu

Image of the Day: Xenopus Pigment

Researchers recently used CRISPR single-guide RNAs to alter genes involved in pigmentation in frog embryos.

May 18, 2018
The Scientist Staff

Embryo at the neurula stage, with tracer localization (green) and pigment loss on the right side of the developing embryo. (Bottom) A more developed embryo, also displaying tracer localization and pigment loss on its right side. VANJA KRNETA-STANKIC


single frog can come with only half its colors. At a workshop on the African clawed frog (Xenopus laevis) and CRISPR applications at the MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, researchers used single-guide RNAs (sgRNAs) to alter pigment genes in the embryos of the amphibian. The scientists injected slc45a2-sgRNA, Cas9 protein and Alexa 488:dextran tracer into one cell of a two-cell embryo. The treatment resulted in half of the embryo lacking pigment. The researchers describe the procedure they used in a study published in February in Genetics


B. Delay et al., “Tissue-specific gene inactivation in Xenopus laevis: knockout of Ihx1 in the kidney with CRISPR/Cas9,” Genetics, doi:10.1534/genetics.117.300468, 2018.


Correction (May 18): An earlier version of this article erroneously referred to MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciencesas the University of Texas Graduate School of Biomedical Sciences. The Scientist regrets this error.

January 2019

Cannabis on Board

Research suggests ill effects of cannabinoids in the womb

Marketplace

Sponsored Product Updates

FORMULATRIX® digital PCR technology to be acquired by QIAGEN
FORMULATRIX® digital PCR technology to be acquired by QIAGEN
FORMULATRIX has announced that their digital PCR assets, including the CONSTELLATION® series of instruments, is being acquired by QIAGEN N.V. (NYSE: QGEN, Frankfurt Stock Exchange: QIA) for up to $260 million ($125 million upfront payment and $135 million of milestones).  QIAGEN has announced plans for a global launch in 2020 of a new series of digital PCR platforms that utilize the advanced dPCR technology developed by FORMULATRIX combined with QIAGEN’s expertise in assay development and automation.
Application of CRISPR/Cas to the Generation of Genetically Engineered Mice
Application of CRISPR/Cas to the Generation of Genetically Engineered Mice
With this application note from Taconic, learn about the power that the CRISPR/Cas system has to revolutionize the field of custom mouse model generation!
Translational Models of Obesity, Dysmetabolism, Diabetes, and Complications
Translational Models of Obesity, Dysmetabolism, Diabetes, and Complications
This webinar, from Crown Bioscience, presents a unique continuum of translational dysmetabolic platforms that more closely mimic human disease. Learn about using next-generation rodent and spontaneously diabetic non-human primate models to accurately model human-relevant disease progression and complications related to obesity and diabetes here!
BiochemAR: an augmented reality app for easy visualization of virtual 3D molecular models
BiochemAR: an augmented reality app for easy visualization of virtual 3D molecular models
Have you played Pokemon Go? Then you've used Augmented Reality (AR) technology! AR technology holds substantial promise and potential for providing a low-cost, easy to use digital platform for the manipulation of virtual 3D objects, including 3D models of biological macromolecules.