The Incucyte® CX3 Live‑Cell Analysis System is designed to give researchers clear, reliable insights into the behaviour of living cells over time, particularly when working with increasingly complex 3D biological models. Built around advanced spinning‑disk confocal optics, the CX3 enables high‑quality imaging within a stable incubator environment, allowing users to follow cellular changes continuously without disturbing their cultures. This combination of confocal‑style clarity and real‑time acquisition makes the system a strong fit for laboratories aiming to capture subtle structural details and dynamic responses that traditional endpoint methods may miss.
The CX3 is especially valuable for teams working with spheroids, organoids, and other thick, multilayered samples. These models often present challenges in contrast and resolution, yet the CX3’s optical design helps reveal features that would otherwise be obscured in standard widefield images. Whether tracking morphology, measuring growth patterns, or observing treatment effects, the system produces consistent, publication‑ready images that support confident interpretation of results.
High‑throughput capability is a core part of the CX3’s design. The system can run up to six microplates simultaneously, enabling long‑term, multiparameter studies while reducing bottlenecks in shared lab environments. This capacity allows researchers to run parallel assays, compare multiple conditions at once, and maintain productivity across extended experiment timelines. The instrument’s compatibility with a wide set of vessels—including microplates from 6‑ to 384‑well formats, flasks, dishes, and slides—adds flexibility for labs working across different model types and assay designs.
To support smooth experimental workflows, the CX3 is paired with intuitive software that simplifies image acquisition and data analysis. Researchers can configure assays quickly using guided setup tools and automated acquisition features, while built‑in kinetic analysis provides immediate, quantitative insight into how cells behave over hours, days, or weeks. This streamlined approach reduces the reliance on manual image handling and helps users convert large datasets into meaningful visual and numerical outputs.
A suite of optional software modules further expands the system’s capabilities. These include cell‑by‑cell measurements, label‑free imaging, AI‑driven nuclei detection, 3D object classification, and application‑specific tools for scratch wound, chemotaxis, neuronal activity, and more. This modular structure gives labs the freedom to tailor the system to current needs while maintaining adaptability for future projects or new scientific questions.
Behind the scenes, the CX3 incorporates robust computing and data‑management features designed to handle the large volumes of image data generated during long‑term experiments. The controller includes substantial onboard storage, with options for expansion, and supports remote access so users can monitor experiments, review results, and schedule imaging from any networked workstation. This makes the platform well suited for busy, multi‑user environments.
Designed for researchers in areas such as 3D cell culture, immuno‑oncology, disease modelling, and translational science, the Incucyte® CX3 Live‑Cell Analysis System offers a balanced combination of clear imaging, scalable throughput, and software tools that make continuous live‑cell analysis more accessible. By bringing clarity to complex biological systems, the CX3 helps scientists generate deeper insights and make faster, more informed experimental decisions.
| Specification | Value |
| Optical System | 3 Fluorescence Channels, Optical Module - Green/Orange/NIR, Objectives 4x,10x,20x, Objective automation - Automated turret |
| Imaging Modes | Spinning Disc Confocal, Widefield Fluorescence, Label-free HD Phase Imaging, Brightfield |
| Spinning Disc | 25 µm pinhole |
| Multi-UserSupport | Remote network Capability, Unlimited free user licenses, Independent vessel scheduling, multi-objective scheduling |
| Incubation | Incubator size >250L required, Incubation to 42ºC, |
| Software Module Capability | Incucyte® Spheroid, Incucyte® Organoid,Incucyte® Cell-by-Cell, Incucyte® Advanced Label-Free, Incucyte® AI Cell Health, Incucyte® ScratchWound*,Incucyte® Chemotaxis*, Incucyte® Neurotrack*, Incucyte® Neuronal Activity*, Incucyte® 21 CFR Part 11*, Incucyte® AI Nuclei Detection Software Module, Incucyte® 3D Object Classification |
| Capacity and Vessel Compatibility | Interchangeable Trays 3 Microplate Capacity 6 Microplate Compatibility 6, 12, 24, 48, 96 and 384-well Flask Compatibility T-25, T-75, T-100, T-175, T-225 Other Compatibility Dishes and slides |
| Computing and Storage | Operating System - Windows 10 IoT Enterprise LTSC |
