Infographic: Visualizing Gene Expression

STARmap reveals expression levels of multiple genes within thick tissue sections.

Ruth Williams
Sep 30, 2018

To visualize gene expression in a thick section of brain tissue with various cell types, RNA targets (top) are amplified to create multiple cDNA copies, each containing a barcode specific to the particular gene.

© george retseck

Acrylamide is then added to the tissue and cross-linked to the cDNA amplicons.

© george retseck

 From the resulting hydrogel, fats and proteins are removed to leave a clear tissue within which the barcodes can be sequenced.

© george retseck

Read the full story