Menu

Infographic: How to Make a Brain Organoid

Mini-brains can be grown in culture or printed.

Aug 1, 2018
Ashley Yeager
THE SCIENTIST STAFF

To grow brain organoids, researchers have traditionally cultured human induced pluripotent stem cells (iPSCs), which develop into clumps of tissue with embryonic features. That tissue is then bathed in proteins to spur the development of nervous-system progenitor cells, which are put into nutrient-containing oil droplets and floated in a spinning bioreactor. After 10 days, neurons begin to form, and in about a month, the neurons begin to spontaneously arrange themselves into different regions that mimic an intact brain. 

To accelerate this process and gain more control over the arrangement of cells in the organoid, researchers have started using 3-D printers. The cells start in a hydrogel-based bio-ink, which is then printed into oil droplets surrounded by a lipid layer. Using separate nozzles, the printer can arrange different cell types in specific patterns. Once printed, the cellular constructs can be transferred to a liquid medium so they can continue to grow in culture. An organoid can be created in this way in just five minutes.


Construction timeSizeSurvival timeCell arrangementImplanted in rodents
Classic brain organoid protocol30 daysUp to 4 mm6–12 monthsSpontaneousYes
3-D printed cerebral tissue approach5 minutes1 mmUp to 2 months, so farPrecise patternsNot yet

Read the full story.

September 2018

The Muscle Issue

The dynamic tissue reveals its secrets

Marketplace

Sponsored Product Updates

Enabling Genomics-Guided Precision Medicine

Enabling Genomics-Guided Precision Medicine

Download this eBook from Qiagen to learn more about the promise of precision medicine and how QCITM Interpret can help deliver better care with better knowledge.

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Download this white paper from Bertin Technologies to learn how to extract and analyze lipid samples from various models!

Bio-Rad Launches CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin for Process Protein Purification

Bio-Rad Launches CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin for Process Protein Purification

Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), a global leader of life science research and clinical diagnostic products, today announced the launch of two new chromatography media for process protein purification: CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin.

Immunophenotypic Analysis of Human Blood Leukocyte Subsets

Immunophenotypic Analysis of Human Blood Leukocyte Subsets

Download this application note from ACEA Biosciences, Inc., to find out how to perform an immunophenotypic analysis of a human blood sample utilizing 13 fluorescent markers using a compact benchtop flow cytometer equipped with 3 lasers!