Infographic: Combo Method of Stem Cell Generation

Simultaneous exposure to reprogramming and gene-editing plasmids efficiently produces edited pluripotent colonies.

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Ruth Williams

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Nov 30, 2017

Plasmids encoding reprogramming factors and plasmids encoding gene-editing machinery are transfected together into fibroblast cells. Approximately three weeks later, induced pluripotent stem cell (iPSC) colonies grown from single cells are apparent. These clones can be individually picked from the dish for further isolated growth and study.

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