Menu

Infographic: Piecing the Cholesterol Puzzle

How a rare disease led to an understanding of the basics of cholesterol regulation.

May 1, 2018
Diana Kwon

See larger version: PDF© CATHERINE DELPHIA

Research into familial hypercholesterolemia (FH), a rare disease in which the body is unable to rid itself of excess low-density lipoproteins (LDLs), provided fundamental insights into cholesterol metabolism. In 1974, geneticist Michael Brown and biochemist Joseph Goldstein reported that LDLs are less likely to bind to cells from FH patients than to cells from healthy individuals. This provided the first evidence that LDL receptors existed on the cell surface and set the stage for later work by Goldstein, Brown, and their colleagues to describe the ways that cholesterol derived from LDL helps regulate the production of the fatty molecule within the cell.

LDL receptors are first synthesized in the endoplasmic reticulum (1). After maturing in the Golgi apparatus, the receptors are transported in vesicles to the cell membrane (2). Once an LDL molecule has bound to the receptor (3), the complex is endocytosed into the cell (4). Then, the resulting endosome is split, and the LDL is gobbled up by a lysosome while the receptor returns back to the cell surface (5). In the lysosome, LDL is degraded into cholesterol and amino acids (6). Increased levels of cholesterol in the cell suppress the transcription of the gene encoding HMG-CoA reductase, a key enzyme required for cholesterol synthesis within the body (7), and the production of more LDL receptors.
 

September 2018

The Muscle Issue

The dynamic tissue reveals its secrets

Marketplace

Sponsored Product Updates

StemExpress LeukopakâNow Available in Frozen Format

StemExpress LeukopakâNow Available in Frozen Format

StemExpress, a Folsom, California based leading supplier of human biospecimens, announces the release of frozen Peripheral Blood Leukopaks. Leukopaks provide an enriched source of peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells that can be used in a variety of downstream cell-based applications.

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

Vector Laboratories, a leader in the development and manufacture of labeling and detection reagents for biomedical research, introduces VECTASHIELD® Vibrance™ – antifade mounting media that delivers significant improvements to the immunofluorescence workflow.

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Download this white paper from Bertin Technologies to learn how to extract and analyze lipid samples from various models!

Bio-Rad Launches CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin for Process Protein Purification

Bio-Rad Launches CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin for Process Protein Purification

Bio-Rad Laboratories, Inc. (NYSE: BIO and BIOb), a global leader of life science research and clinical diagnostic products, today announced the launch of two new chromatography media for process protein purification: CHT Ceramic Hydroxyapatite XT Media and Nuvia HP-Q Resin.