REVEALING RIBOSWITCHES: RNA is isolated from bacteria and tagged with a 3’ adapter, to which a complimentary oligonucleotide binds for reverse transcription and sequencing. Sequence analysis then reveals those transcripts that have been fully transcribed versus those prematurely truncated. The reproducible presence of similarly truncated transcripts for a given gene suggests the presence of a riboswitch in the mRNA.© GEORGE RETSECKRead the full story.
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