Troubleshooting discovery and validation of protein biomarkers for cancer.

Making the most of mass spectrometry imaging.

How to troubleshoot sample preparation for cryo-electron microscopy, an up-and-coming structural biology technique.

How to modify your tools to prevent pain at the bench.

What beginners need to know as they dive into studies on pluripotent cells.

How to put FRET biosensors to work for you when tracking cell signaling.

Protein-protein interaction assays for all occasions.

Avoiding the pitfalls of medium-throughput SNP detection.

Credit: Courtesy of Phillip Zamore, University of Massachusetts Medical School, and Science." /> Credit: Courtesy of Phillip Zamore, University of Massachusetts Medical School, and Science. Researcher: Phillip Zamore, Gretchen Stone Cook Professor of Biomedical Sciences, University of Massachusetts Medical School, Worcester Project:

1. Try orthagonal approaches Every assay has strengths and weaknesses, so it's a good idea to try more than one, if possible. ChIP-on-chip is significantly cheaper than ChIP-Seq, but unless you tile the entire genome, you will miss any region not represented on your chip. HELP probes only a fraction of the genome, but a genome's worth of testable HpaII fragments will fit on a single array, making analysis relatively inexpensive. (Greally now uses a 1.32-million element Nimblegen array.)

How to conduct your next large-scale epigenetic analysis

Researcher: Richard Young, member, Whitehead Institute for Biomedical Research, Cambridge, Mass. Project: Mapping transcription factor binding across the yeast genome. Problem: Chromatin immunoprecipita

Credit: © 2008 Illumina Inc. All Rights Reserved." /> Credit: © 2008 Illumina Inc. All Rights Reserved. Researcher: Steven Jones, head, Bioinformatics, Genome Sciences Center, British Columbia Cancer Research Center, Vancouver, BC, Canada Project: Mapping transcription-factor binding in interferon-gamma-stimulated and unstimu

Credit: Courtesy of John Greally, Albert Einstein College of Medicine, and PLoS ONE" /> Credit: Courtesy of John Greally, Albert Einstein College of Medicine, and PLoS ONE Researcher: John Greally, associate professor of Molecular Genetics, Albert Einstein College of Medicine, NY Project: Comparing ep

Credit: Courtesy of Matteo Pellegrini, University of California, Los Angeles" /> Credit: Courtesy of Matteo Pellegrini, University of California, Los Angeles Researcher: Matteo Pellegrini, assistant professor of Molecular, Cell and Developmental Biology, University of California, Los Angeles Project:

How to troubleshoot your in-vivo fluorescence imaging studies

C6 glioma cells label with self-illuminating quantum dots conjugated with cell penetrating peptides. Credit: Jianghong Rao, Stanford School of Medicine" />C6 glioma cells label with self-illuminating quantum dots conjugated with cell penetrating peptides. Credit: Jianghong Rao, Stanford School of Medicine User: Jianghong Rao, Assistant Professor in Radiology, Stanford University

How to find your way around three-dimensional cell culture.

User: Joan Brugge, Harvard Medical School, Cambridge, Mass. Project: Investigating morphogenesis of 3-D structures such as luminal epithelial tissue Related Articles Shaping Up Commerical 3D matrices Tips for setting up 3-D cell cultures Home-made matrix Mixed gel Planar imaging Problem: Cells may behave diffe

Scanning electron micrograph of human fibroblast (gray) intercalating within three-dimensional in vivo-like cell-derived matrix (cyan). Credit: Courtesy of Michal Jarnik and Edna Cukierman / Fox Chase Cancer Center" />Scanning electron micrograph of human fibroblast (gray) intercalating within three-dimensional in vivo-like cell-derived matrix (cyan). Credit: Courtesy of Michal Jarnik and Edn