Lab Tools (old)

Pulling Out Proteins
Kelly Rae Chi | Apr 1, 2009
Troubleshooting discovery and validation of protein biomarkers for cancer.
Mass Spectacle
Jeffrey M. Perkel | Mar 1, 2009
Making the most of mass spectrometry imaging.
Freeze Frame
Jeffrey M. Perkel | Feb 1, 2009
How to troubleshoot sample preparation for cryo-electron microscopy, an up-and-coming structural biology technique.
Let's Get Physical
Kelly Rae Chi | Jan 1, 2009
How to modify your tools to prevent pain at the bench.
Pluripotency for the Masses
Kelly Rae Chi | Dec 1, 2008
What beginners need to know as they dive into studies on pluripotent cells.
Lab Tools: Fret-free FRET
Kelly Rae Chi | Nov 1, 2008
How to put FRET biosensors to work for you when tracking cell signaling.
Lab Tools: Close Encounters
Jeffrey M. Perkel | Oct 1, 2008
Protein-protein interaction assays for all occasions.
Middling Measures
Kelly Rae Chi | Sep 1, 2008
Avoiding the pitfalls of medium-throughput SNP detection.
Modifications Abound
Jeffrey M. Perkel | Aug 1, 2008
How to conduct your next large-scale epigenetic analysis
ChIP-on-chip
Jeffrey M. Perkel | Aug 1, 2008
Researcher: Richard Young, member, Whitehead Institute for Biomedical Research, Cambridge, Mass. Project: Mapping transcription factor binding across the yeast genome. Problem: Chromatin immunoprecipita
ChIP-Sequence
Jeffrey M. Perkel | Aug 1, 2008
Credit: © 2008 Illumina Inc. All Rights Reserved." /> Credit: © 2008 Illumina Inc. All Rights Reserved. Researcher: Steven Jones, head, Bioinformatics, Genome Sciences Center, British Columbia Cancer Research Center, Vancouver, BC, Canada Project: Mapping transcription-factor binding in interferon-gamma-stimulated and unstimu
Enrichment HELP
Jeffrey M. Perkel | Aug 1, 2008
Credit: Courtesy of John Greally, Albert Einstein College of Medicine, and PLoS ONE" /> Credit: Courtesy of John Greally, Albert Einstein College of Medicine, and PLoS ONE Researcher: John Greally, associate professor of Molecular Genetics, Albert Einstein College of Medicine, NY Project: Comparing ep
Single-base detection
Jeffrey M. Perkel | Aug 1, 2008
Credit: Courtesy of Matteo Pellegrini, University of California, Los Angeles" /> Credit: Courtesy of Matteo Pellegrini, University of California, Los Angeles Researcher: Matteo Pellegrini, assistant professor of Molecular, Cell and Developmental Biology, University of California, Los Angeles Project:
Recognizing RNA
Jeffrey M. Perkel | Aug 1, 2008
Credit: Courtesy of Phillip Zamore, University of Massachusetts Medical School, and Science." /> Credit: Courtesy of Phillip Zamore, University of Massachusetts Medical School, and Science. Researcher: Phillip Zamore, Gretchen Stone Cook Professor of Biomedical Sciences, University of Massachusetts Medical School, Worcester Project:
Tips for wide-scale epigenetic detection
Jeffrey M. Perkel | Aug 1, 2008
1. Try orthagonal approaches Every assay has strengths and weaknesses, so it's a good idea to try more than one, if possible. ChIP-on-chip is significantly cheaper than ChIP-Seq, but unless you tile the entire genome, you will miss any region not represented on your chip. HELP probes only a fraction of the genome, but a genome's worth of testable HpaII fragments will fit on a single array, making analysis relatively inexpensive. (Greally now uses a 1.32-million element Nimblegen array.)
In the Live Light
Richard Gaughan | Jul 1, 2008
How to troubleshoot your in-vivo fluorescence imaging studies
Source lighting
Richard Gaughan | Jul 1, 2008
C6 glioma cells label with self-illuminating quantum dots conjugated with cell penetrating peptides. Credit: Jianghong Rao, Stanford School of Medicine" />C6 glioma cells label with self-illuminating quantum dots conjugated with cell penetrating peptides. Credit: Jianghong Rao, Stanford School of Medicine User: Jianghong Rao, Assistant Professor in Radiology, Stanford University
Tips for setting up 3-D cell cultures
Josh P. Roberts | Jun 1, 2008
Choose your substrate. Basement membrane extract (BME) is fine for studying tissue-specific differentiation, because it can approximate the morphology and viscoelasticity of the tissues in extracellular matrices in vivo, explains Valerie Weaver of the University of California, San Francisco. If you're working on invasive tumors, however, try collagen or a similar substra
Shaping Up
Josh P. Roberts | Jun 1, 2008
How to find your way around three-dimensional cell culture.
In stock
Josh P. Roberts | Jun 1, 2008
User: Joan Brugge, Harvard Medical School, Cambridge, Mass. Project: Investigating morphogenesis of 3-D structures such as luminal epithelial tissue Related Articles Shaping Up Commerical 3D matrices Tips for setting up 3-D cell cultures Home-made matrix Mixed gel Planar imaging Problem: Cells may behave diffe