David Muddiman, professor of chemistry, North Carolina State University, Raleigh
Profiling O-linked glycans as biomarkers in patients with ovarian cancer
Developing the chemistry to efficiently cleave O-linked glycans from their peptides in complex biofluids.
After a year of effort, Muddiman has finally figured out how to adapt a standard chemical process called beta-elimination to clip O-linked glycans from proteins in biological fluids ( J Proteome Res, in press). Following purification, he then analyzes these using high-end mass spectrometers to...