Mixed gel

Mammary FSK-3 cells plated onto matrix pads consisting of rat mammary ECM isolated from a nulliparous mouse, treated with 24hr pulse of BrdU and processed for immunohistochemical detection. Credit: Pepper Schedin / University of Colorado Health Sciences Center" />Mammary FSK-3 cells plated onto matrix pads consisting of rat mammary ECM isolated from a nulliparous mouse, treated with 24hr

Josh P. Roberts
May 31, 2008
<figcaption>Mammary FSK-3 cells plated onto matrix pads consisting of rat mammary ECM isolated from a nulliparous mouse, treated with 24hr pulse of BrdU and processed for immunohistochemical detection. Credit: Pepper Schedin / University of Colorado Health Sciences Center</figcaption>
Mammary FSK-3 cells plated onto matrix pads consisting of rat mammary ECM isolated from a nulliparous mouse, treated with 24hr pulse of BrdU and processed for immunohistochemical detection. Credit: Pepper Schedin / University of Colorado Health Sciences Center

User:
Pepper Schedin, University of Colorado Health Sciences Center, Denver

Project:
Relating life-cycle stages (such as pregnancy, lactation, and menopause) in the mammary gland stroma to tumor dormancy and progression

Problem:
Like Cukierman, Schedin looks at interactions of different ECMs with tumors. Schedin, though, extracts ECM from whole tissue; here, the resulting solution is too dilute to polymerize on its own - about 1/10 to 1/20th the concentration of Matrigel. Protocols to concentrate the ECM end up concentrating cellular contaminants along with it.

Solution:
Mixing the extracted ECM solutions 1:1 (volume to...