Pepper Schedin, University of Colorado Health Sciences Center, Denver
Relating life-cycle stages (such as pregnancy, lactation, and menopause) in the mammary gland stroma to tumor dormancy and progression
Like Cukierman, Schedin looks at interactions of different ECMs with tumors. Schedin, though, extracts ECM from whole tissue; here, the resulting solution is too dilute to polymerize on its own - about 1/10 to 1/20th the concentration of Matrigel. Protocols to concentrate the ECM end up concentrating cellular contaminants along with it.
Mixing the extracted ECM solutions 1:1 (volume to...