Long hours of manual pipetting does not need to be the default for ccfDNA extraction. Manual processing of plasma and ccfDNA purification isn't just a time cost, every manual handling step can introduce variability in downstream assays. The Maxwell® Rapid ccfDNA Kit replaces that with a single automated purification run with no sample handling in between. Yields are comparable to most commonly used manual and automated ccfDNA extraction kits and the ccfDNA eluate is of high quality and purity for rare-variant detection. Automate ccfDNA extraction from 48 samples in under 30 minutes on a Maxwell® instrument, or scale to 96 on third-party automation. Circulating cell-free DNA drives liquid biopsy workflows across oncology testing, minimal residual disease monitoring, non-invasive prenatal testing, and transplant surveillance. Analytical assay accuracy and sensitivity depend on the ccfDNA extraction step. Unreliable extraction surfaces later as failed digital PCR reactions, unreliable quantitation, and repeat NGS runs.The Maxwell® Rapid ccfDNA Kit removes the manual preprocessing variable completely. Plasma goes straight into prefilled cartridges with no pretreatment, no reagent prep, no organic solvents. With minimized genomic DNA carryover and no carrier RNA to confound quantitation, this means cleaner input for qPCR, digital PCR, and NGS, and more confidence detecting low-frequency variants. A single protocol handles 1–4ml of plasma ccfDNA with linear recovery, so variable collection volumes do not require a different kit or a different method. Every reagent and consumable is included.
Product Specifications:
| Specification | Value |
| Sample Type | Human plasma (application notes available for serum, urine, CSF, pleural fluid) |
| Sample Input Volume | 1–4 mL plasma (HT format: 4 mL and scalable to 10 mL) |
| Instrument Run Time | Under 30 minutes (Maxwell® RSC/CSC Instruments); Under 45 minutes on KingFisher® instruments (HT) |
| Total Workflow Time | Under 1 hour, plasma to purified ccfDNA |
| Throughput Per Run | 1–16 or 1–48 samples (Maxwell® RSC/CSC Kits); Scalable to 96 samples or more (HT) |
| Elution Volume | 50 µL (Adjustable, 20–80 µL in RUO format) |
| Sample Pretreatment | None required; Maxwell® prefilled cartridges, load and go |
| Extraction Chemistry | Paramagnetic resin, solvent-free (no ethanol or organic solvents) |
| Downstream Compatibility | Digital PCR, qPCR, next-generation sequencing |
| Available Formats | AS1590 Maxwell® RSC Rapid ccfDNA Kit (RUO, 48 preps) AS1580 Maxwell® CSC Rapid ccfDNA Kit (CE-Marked, IVD, 48 preps) A6080 Maxwell® HT Rapid ccfDNA Kit (96 preps) |
