D.D Ho, T. Moudgil, M. Alam, "Quantitation of human immunodeficiency virus type 1 in the blood of infected persons," New England Jpurnal of Medicine, 321, 1621-25, 14 December 1989.
David D. Ho (University of California, Los Angeles, School of Medicine): "This paper suumarizes results from our study to quantify HIV-1 in the blood of infected persons at different stages of disease. The findings showed that viral burden increases with disease progression and that HIV-1 infection should be viewed as an active persistent infection rather than a latent one. Overall, the amount of virus carried in the blood was several orders of magnitude higher than a previously believed. This finding would suggest that direct depletion of CD4+ cells by HIV-1 is a feasable mechanism.
"The quantitative methods described in this paper will be useful in monitoring the clinical efficacy of novel therapeutic agents developed for AIDS and AIDS-related complex."