Menu

Time-Lapse on the Cheap

A PhD student jury-rigs a microscopy system for high-throughput cell motility assays.

Feb 1, 2015
Ruth Williams

LOOK AT 'EM GO: The LOCOMOTIS microscopy rig includes three inexpensive microscopes, each mounted below a sample in a dish. Software records data coming in from the three scopes simultaneously. A heated cable in the sample chamber allows for temperature manipulation.© GEORGE RETSECK

Adam Lynch, a doctoral student at Brunel University London, studies the effects of waterway pollutants on the physiology of the freshwater snail Biomphalaria glabrata. As part of his thesis, he planned to observe—using time-lapse imaging—how a variety of chemicals affect the motility of the snail’s immune cells.

To perform such imaging, researchers traditionally placed a cell culture dish on an inverted microscope and took photos with a software-driven digital camera at regular time intervals. To look at several experimental conditions in parallel—which Lynch hoped to do—would thus require several microscopes, or an automated stage fitted to one microscope that enabled precision movements of a multiwell culture dish.

Lynch did not have access to either, but he did have access to the Internet. There he bought three cheap microscopes, each of which could transmit digital images to his laptop, and found computer programmer Junian Triajianto, an independent coder who wrote software allowing image recording from all three microscopes at once. The result is LOCOMOTIS—a Low-Cost Motility Tracking System—comprised of the three amateur mini-microscopes, some adjustable kitchen-cabinet legs, a soil warmer, and a desk lamp. Lynch built a case for the microscopes and a temperature-controlled box for the samples, and then watched as the snail immune cells moved. The system provides an extremely economical alternative to expensive professional microscope setups for observing cell movements.

LOCOMOTIS “won’t replace a serious microscope” for analyzing fine cellular details, says cell-migration expert Robert Insall of the Cancer Research UK Beatson Institute, but as a basic screen for cell motility “it’s fantastic. [It allows] people in labs that have traditionally done biochemical assays to now actually screen for cell migration just as easily.” (PLOS ONE, 9:e103547, 2014)

TECHNIQUE APPROX. COST NUMBER OF PARALLEL SAMPLES MAX SPATIAL RESOLUTION IMAGE QUALITY
Inverted microscope with automated stage and temperature control $100,000 Limited only by size of stage and time interval between images (must allow time to move between wells and autofocus camera) Approximately 1–2 µm High: mechanisms exist to create contrast, allowing the invisible to become visible, e.g., differential interference contrast or phase-contrast systems
LOCOMOTIS $250 Three At least 10 µm Limited: uneven lighting and no mechanism for creating contrast

 

September 2018

The Muscle Issue

The dynamic tissue reveals its secrets

Marketplace

Sponsored Product Updates

Horizon Discovery introduces Myeloid DNA Reference Standard to support genetic testing of leukemia

Horizon Discovery introduces Myeloid DNA Reference Standard to support genetic testing of leukemia

Horizon Discovery Group plc, a global leader in gene editing and gene modulation technologies, today announced the launch of its Myeloid DNA Reference Standard. The first-to-market large cell-line derived myeloid cancer reference standard designed enables faster, more reliable and more cost-effective assay validation, to support the market in bringing routine testing into practice.

StemExpress LeukopakâNow Available in Frozen Format

StemExpress LeukopakâNow Available in Frozen Format

StemExpress, a Folsom, California based leading supplier of human biospecimens, announces the release of frozen Peripheral Blood Leukopaks. Leukopaks provide an enriched source of peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells that can be used in a variety of downstream cell-based applications.

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

New Antifade Mounting Media from Vector Laboratories Enhances Immunofluorescence Applications

Vector Laboratories, a leader in the development and manufacture of labeling and detection reagents for biomedical research, introduces VECTASHIELD® Vibrance™ – antifade mounting media that delivers significant improvements to the immunofluorescence workflow.

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Best Practices for Sample Preparation and Lipid Extraction from Various Samples

Download this white paper from Bertin Technologies to learn how to extract and analyze lipid samples from various models!