And Fixation/Permeabilization Kits Pharmingen (San Diego, CA) provides a wide variety of cytokine and chemokine specific antibodies directly labeled with FITC or PE for detection of intracellular cytokines or chemokines by Flow Cytometry. These antibodies are valuable tools for analysis of the roles played by different cytokines in mediation of inflammatory and specific immune responses. To enhance the utility of these cytokine or chemokine specific antibodies, Pharmingen has recently introduced three kits to simplify the fixation and permeabilization of cells for immunofluorescent staining studies of intracellular cytokines and chemokines. All three of these kits provide one-step fixation and permeabilization of cells.

The Cytofix/CytopermTM Kit provides a fixation/permeabilization solution and an antibody diluent / wash solution. The Cytofix/CytopermTM Plus (with GolgiStopTM) Kit provides these two solutions plus a protein transport inhibitor (Monensin) for the treatment of freshly explanted or cultured cells to promote intracytoplasmic cytokine accumulation. The...

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