New Products

Dialog Information Services Issues Nuclear Science Abstracts On CD-ROM Biotecx Laboratories Introduces IMMULAN Purification Kits Nalge Announces A PES Membrane For Tissue - Culture Applications Geno Technology Launches Gene CAPSULE For DNA Extraction Phycoerythrin - Cy5 Conjugated Antibodies For Flow Cytometry From AMAC Labsystems Unveils Microplate Luminometers Elkay Products Inc. Releases New Autotubes Abstracts On CD-ROM Dialog Information Services Inc. of Palo Alto, Calif., has released DIALOG OnDisc Nuclear Science Abstracts (NSA), a CD-ROM database containing nearly 1 million records from the 1948-76 volumes of Nuclear Science Abstracts. The database provides bibliographic references for books, journal articles, conference proceedings, technical reports, patents, dissertations, engineering drawings, and other documents of interest to professionals in nuclear research, development, and production. Abstracts are included for approximately 25 percent of the references. NSA provides background data for current research in nuclear science areas: reactor technology; particle accelerators; nuclear materials and waste management; radiation effects; isotope and radiation source technology; metals, ceramics, and other materials; and fusion energy. The NSA database precedes Dialog's OnDisc DOE (Department of Energy) Energy Science and Technology, which covers 1974 to the present. Geno Technology Inc. of St. Louis announces the release of GeneCAPSULE, a new, single-use device for rapid DNA extraction from gels. Using electroelution and requiring no additional equipment, the new product takes as little as 90 seconds to recover DNA in amounts ranging from 10 to 30 ml, and less than 60 seconds to recover 1,000 bp, according to the company. The recovered DNA is ready for ligation, restriction-enzyme digestion, sequencing, amplification, random priming, nick translation, and other enzymatic reactions. GeneCAPSULE is supplied as a 54-unit kit. Houston-based Biotecx Laboratories Inc. has introduced IMMULAN immune column separation kits for T cells and T-cell subpopulations. The kits facilitate affinity chromatography separation and selection of rat, mouse, human, cat, dog, and monkey T cells derived from peripheral blood, lymph nodes, spleen, thymus, and other tissues. Through selective chemical elimination, users can separate CD4+ and CD8+ T cells in high yield. The purity of isolated T cells is said to range from 90 percent to 99 percent; that of subpopulations is said to be from 93 percent to 98 percent, with virtually all of the B-cell population and macrophages removed. The kits allow for 30 T-cell purifications and 20 T-cell subpopulation separations. They use immunoselective microparticles coated with polyclonal IgG (H and L chains) and include plastic, adjustment-free columns accommodating standard 50 ml centrifuge tubes as well as pretested, ready- to-use reagents. Cytometry From AMAC AMAC Inc., headquartered in Westbrook, Maine, has added PE- Cy5 conjugated antibodies to its line of products for flow cytometry: CD3, CD4, CD19, CD34, and CD45, as well as IgG1 phycoerythrin-Cy5, all available in liquid form, in vials that will last for 100 tests. This fluoro-chrome allows for multiparameter analysis of lymphocyte populations with other fluorochromes and a single-laser flow cytometer. AMAC's fluorescein isothiocyanate (FITC), phycoerythrin, and phycoerythrin-Cy5 are all excited at 488 nm, with FITC emitting at 525 nm, phycoerythrin at 575 nm, and phycoerythrin-Cy5 at 670 nm. The majority of photons emitted by phycoerythrin in the tandem fluorochrome PE-Cy5 (greater than 95 percent) are captured by Cy5, which avoids light contamination in the phycoerythrin detector by the PE-Cy5 dye spectrum. The phycoerythrin-Cy5 molecule is designed to yield a very high level of fluorescence intensity. Elkay Products Inc. of Shrewsbury, Mass., offers autotubes for use in HTLV-III testing, polymerase chain reaction cell- harvesting procedures, cell-uptake studies, receptor-binding assays, radioimmunoassays, and enzyme immunoassays. They can also be used for freezer storage of blood bank samples, as well as laboratory transport and storage of specimens and reagents. The autotubes provide convenient, extra-volume capacity for sample predilution prior to transfer into a microplate for analysis. They are packaged in a 96-position, stackable 8 o 12 rack. Standard 9 mm on-center spacing of tubes is used for the Autotube 1.2 ml rack, making it compatible with leading multichannel pipettors and autosampling devices. Each rack of tubes contains a removable, 96-place identification card for recording tube locations for each sample. The tubes, rack, and cover are made from 100 percent virgin polypropylene, are fully autoclavable, and can be stored to -85oC. Sterile or nonsterile polyethylene plug caps are supplied on strips (eight caps per strip), which are not autoclavable; the caps help prevent sample contamination during mixing and storage. Labsystems, located in Needham Heights, Mass., has announced the release of three new micro-plate luminometers, all robot-compatible and computer- controllable. The Luminoskan EL is designed for use with enhanced luminescence, or "glow," reagents. The basic system--without injectors, temperature control, or a mixer--offers a choice of eight measurement modes and computer controls. The Luminoskan RT has two injectors and an orbital mixer as standard features. It is suited for applications requiring reagent addition without the need for temperature control. The Luminoskan RS includes bilevel temperature control ranging from 20o to 43oC, orbital mixing, and two injectors; two other injectors may be added as an option. Applications Nalge Co. of Rochester, N.Y., has introduced a line of filtration products featuring a new polyethersulfone (PES) membrane for a variety of tissue-culture applications. PES, an inherently hydrophilic membrane, offers faster flow rates and fluid throughputs than other sterilizing-grade filtration membranes, according to the company. This is said to reduce the number of filter changes, thereby cutting filtration time and costs. In addition, the company says that PES results in lower extractables and protein binding and better chemical resistance than occur with acetates, nitrate, or nylon membranes. The PES membrane is available on NALGENE 50 mm and 90 mm filter units, bottle cap filters, and syringe filters, which are radiation sterilized and certified sterile, noncytotoxic, and nonpyrogenic.

New Products

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