Worthington Biochemical Corp. of Freehold, N.J., offers a cell isolation kit called the Cardiomyocyte Isolation System. The kit contains materials and directions for five separate tissue dissociations of up to 12 hearts each. Individual vials contain Hank's Balanced Salt Solution, Worthington Purified Trypsin, Soybean Trypsin Inhibitor, Purified Collagenase, and Leibovitz L-15 powdered media. Also included with instructions are five Falcon cell strainers and a phenol red color card for checking pH. All kits use purified enzyme preparations, rather than crude; according to the company, this ensures additional consistency and yield of viable cells at the bench. Additionally, all kits have been use-tested on neonatal rat hearts.
Unisyn Technologies, based in San Diego, has released its Cell-Pharm System 1000 research- to pilot-scale cell culture system for producing biomolecules. Designed with hollow fiber bioreactor technology, the system provides highly concentrated, debris-free supernatants to simplify downstream purification, improve yields, and broaden cell production...

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