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Multifunction Electrophoresis System Eliminates Excess Equipment

Researchers who analyze and isolate specific proteins and nucleic acids often find that gel electrophoresis is the method of choice for these experiments. Proteins or nucleic acid mixtures are separated into individual species based on differences in molecular weight as they migrate through an acrylamide or agarose gel in the presence of an electric field. In addition to serving as an analytical tool, individual proteins and nucleic acids, once separated, can then be isolated in pure form by

Wendy Wilson Sheridan

Researchers who analyze and isolate specific proteins and nucleic acids often find that gel electrophoresis is the method of choice for these experiments. Proteins or nucleic acid mixtures are separated into individual species based on differences in molecular weight as they migrate through an acrylamide or agarose gel in the presence of an electric field. In addition to serving as an analytical tool, individual proteins and nucleic acids, once separated, can then be isolated in pure form by the electroelution of a specific band from the gel into an aqueous phase. While these procedures typically require multiple steps and more than one piece of equipment, Isco Inc., based in Lincoln, Neb., has developed a multifunctional electrophoresis system in which electrophoretic separation and recovery of specific molecules by electroelution are performed in the same apparatus.

The device, priced at $295, will be available next month. The system includes a horizontal buffer...

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