For Detecting Pathogen-Specific PCR Products The DNA Catcher Plate System includes a line of 96-well oligo plate assays for the detection of pathogen-specific polymerase chain reaction (PCR) products. Kits include HIV, HBV, HCV, HCMV, HSV, VZV, HHV-6, Borrelia burgdorferi, and custom applications. The company's method for attaching a high-density capture probe to a 96-well microtiter plate allows single-copy detection of amplified DNA in a reverse dot blot format. Amplification of the target is performed by using biotinylated primers included in the kit. The amplified product is transferred directly to the plate, followed by a 30-minute hybridization. Streptavidin conjugate is then added for 10 minutes and the plate is developed with TMB substrate. The plates can be read on a standard plate reader.
The new line is available in a variety of platform sizes and load capacities. Users can choose from five specific motions: vibrating, orbital,...
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