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Three new DNA markers have been developed to allow sizing and quantitation of linear and supercoiled, double-stranded DNA. The Kb DNA Ladder contains 15 linear fragments from 250 bp to 12 kb. Bands are produced in 1,000-bp increments from 1 kb to 10 kb. The ends of each fragment have 5_ overhangs and may be radiolabeled with a-labeled dATP, dTTP, or dUTP. Alternatively, the ladder may be radiolabeled by exchanging the 5_ phosphate u

The Scientist Staff

Three new DNA markers have been developed to allow sizing and quantitation of linear and supercoiled, double-stranded DNA. The Kb DNA Ladder contains 15 linear fragments from 250 bp to 12 kb. Bands are produced in 1,000-bp increments from 1 kb to 10 kb. The ends of each fragment have 5_ overhangs and may be radiolabeled with a-labeled dATP, dTTP, or dUTP. Alternatively, the ladder may be radiolabeled by exchanging the 5_ phosphate using T4 polynucleotide kinase and g-labeled rATP. The Illuminator Kb DNA is a version of the Kb DNA Ladder that has been prelabeled with fluor-12-dUTP for use with the Illuminator nonradioactive detection system. The Supercoiled DNA Ladder produces bands from 2 kb to 10 kb at 1-kb increments.
Stratagene, La Jolla, CA
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