Department of Microbiology & Immunology
University of Illinois

  • A surface glycoprotein that is anchored via a lipid to the trypanosome cell membrane is transferred in vitro to red blood cell membranes, sensitizing the latter to attack by anti-trypanosome antibodies.

    M.R. Rifkin, F.R. Landsberger, "Trypanosome variant surface glycoprotein transfer to target membranes: a model for the pathogenesis of trypanosomiasis," PNAS, 87, 801-5, January 1990. (Rockefeller University, New York)

  • Gene fusions encoding reporter enzymes such as alkaline phosphatase and á-galactosidase are a means for following the twisting of membrane proteins back and forth across the bacterial cell membrane. A review and new transposons developed for this technique should extend its utility.

    C. Manoil, J.J. Mekalanos, J. Beckwith, "Alkaline phosphatase fusions: sensors of subcellular location," Journal of Bacteriology, 172, 515-18, February 1990. (University of Washington, Seattle; Harvard Medical School, Boston) C. Manoil, "Analysis of protein localization by use...

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