M. Gray, S.M. Honigberg, "Effect of chromosomal locus, GC content and length of homology on PCR-mediated targeted gene replacement in Saccharomyces," Nucleic Acids Research, 29:5156-62, Dec. 15, 2001.

F1000 Rating: Recommended

"While gene knockouts or replacements are easily and commonly done in yeast, the targeting efficiency is quite variable. The authors have systematically evaluated key parameters and found that a GC content of at least 40% in the regions of homology improves targeting efficiency. Surprisingly, changing the length of targeting homology from 40 to 60 bp had little effect."

—Janet Lindsley,
University of Utah, US

Genomes & Genomics

R. Tompa et al., "Genome-wide profiling of DNA methylation reveals transposon targets of CHROMOMETHYLASE3," Current Biology,12:65-8, Jan. 8, 2002.

F1000 Rating: Recommended

"A new technique can be used to monitor cytosine methylation across many genomic sites using microarrays. Using this technique, the authors investigate the targets...

Interested in reading more?

Magaizne Cover

Become a Member of

Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member?