In the latest of a series of papers from his lab, Stefan Hell and researchers of the Max Planck Institute for Biophysical Chemistry in Germany detail an approach to microscopy that achieves a resolution of half the wavelength of light.
“What drives this work is the availability of genetically encoded fluorophores, like GFP and its descendents,” says Dr. Steven G. Boxer, a member of the Faculty of 1000 and a chemistry professor at Stanford University. “Hell is using an approach that can offer great advantages in the context of live cell microscopy, looking deep inside a cell,” he says. “It’s a global question that everyone is interested in: whether one can see the location of components during a particular cellular function, and if you perturb the function, can you see what changes inside the cell? All microscopy methods are trying to assemble a snapshot and then ultimately get movies of cell function in as much detail as possible? This development is a significant step in imaging complicated biological specimens with high spatial resolution.”
1. G. Donnert, “Two-color far-field fluorescence nanoscopy,” Biophys J: Biophys Lett, 92(8):L67?9, April 15, 2007.
These papers were selected from multiple disciplines from the Faculty of 1000, a web-based literature awareness tool (www.f1000biology.com).