ADVERTISEMENT
ADVERTISEMENT

West Nile Virus Triggers Apoptosis, Smelling Good May Cost Too Much, Science Seen

West Nile Virus Triggers Apoptosis; Smelling Good May Cost Too Much; Three Green Mice... Three Green Mice Courtesy of NASA IMMUNOLOGY | West Nile Virus Triggers Apoptosis Even as West Nile virus (WNV) spreads, researchers still know little about its infective choreography. To track the steps, David B. Weiner, an associate professor of pathology and laboratory medicine at the University of Pennsylvania, and colleagues marked WNV capsid protein with green fluorescent protein (GFP) and trans

Ricki Lewis

West Nile Virus Triggers Apoptosis; Smelling Good May Cost Too Much; Three Green Mice... Three Green Mice


Courtesy of NASA

IMMUNOLOGY | West Nile Virus Triggers Apoptosis
Even as West Nile virus (WNV) spreads, researchers still know little about its infective choreography. To track the steps, David B. Weiner, an associate professor of pathology and laboratory medicine at the University of Pennsylvania, and colleagues marked WNV capsid protein with green fluorescent protein (GFP) and transfected cultured human cells and murine muscle and brain. GFP-marked capsids, which form the viral shell, disrupted mitochondrial membranes, triggering apoptosis and inflammation. Deleting part of the capsid gene stunted the capsid protein and dampened the death signal (J.S. Yang et al., "Induction of inflammation by West Nile virus capsid through the caspase-9 apoptotic pathway," Emerg Infect Dis, 8:1379-84, December 2002).

"We mapped the apoptotic part of the protein to the carboxyl terminus," Weiner says. "This...

Interested in reading more?

Become a Member of

Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!
Already a member?
ADVERTISEMENT