Actin comet tails' tale

Measuring the force generated by actin-based intracellular bacterial motility.

Andrea Rinaldi(rinaldi@unica.it)
May 6, 2003

Spatially controlled polymerization of the cytoskeleton protein actin generates the motile force required for biological processes such as the formation of cellular protrusions like lamellipodia, cancer cell metastases, and the intracellular movements of bacterial and viral pathogens within the cytosol of infected cells. In the May 5 PNAS, Paula Giardini and colleagues at Stanford University School of Medicine describe a model system enabling a qualitative and quantitative analysis of the mechanism of force generation in this poorly understood form of biological motility (PNAS, DOI:10.1073/pnas.1031670100, May 5, 2003).

Giardini et al. developed a membrane-based reconstituted system in which large artificial lipid vesicles coated with the protein ActA from the bacterium Listeria monocytogenes — a facultative intracellular pathogen capable of rapid movement through the host cell cytoplasm — were propelled by actin polymerization in cytoplasmic extracts. The model closely mimics in vivo conditions. To move within the cytosol of...

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