Barcode screening

An international project is replacing each yeast open reading frame (ORF) with a drug-resistance cassette containing two 20-mer oligonucleotide 'barcodes' that can be used as hybridization tags for each gene. In the November 8 Sciencexpress, Siew Loon Ooi and colleagues describe the use of this resource to screen for mutants defective in nonhomologous end-joining (NHEJ) (10.1126/science.1065672).They used a transformation-based plasmid-repair assay to screen for NHEJ activity. They prepared hapl

Jonathan Weitzman(jonathanweitzman@hotmail.com)
Nov 11, 2001

An international project is replacing each yeast open reading frame (ORF) with a drug-resistance cassette containing two 20-mer oligonucleotide 'barcodes' that can be used as hybridization tags for each gene. In the November 8 Sciencexpress, Siew Loon Ooi and colleagues describe the use of this resource to screen for mutants defective in nonhomologous end-joining (NHEJ) (10.1126/science.1065672).

They used a transformation-based plasmid-repair assay to screen for NHEJ activity. They prepared haploid and homozygous diploid deletion pools which were then transformed with circular or linearized plasmids. Ooi et al. hybridized DNA isolated from the pools to microarrays containing the barcode tags, and thus identified known and novel genes associated with the NHEJ pathway.

This study highlights the effective application of hybridization barcode tags to screen pools of thousands of mutants in parralel.

Interested in reading more?

Become a Member of

Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!
Already a member?