The search is on to identify and locate neural stem cells (NSCs). In the August 16 issue of
They performed a series of enrichment steps to purify NSCs from the ependymal and subventricular zones of the lateral ventricular walls. Flow cytometry was used to select a population expressing low amounts of peanut agglutinin (PNAlo) and heat-stable antigen (HSAlo). Although these cells represent just 0.27% of the unsorted population, they contain predominantly NSCs and account for 63.2% of the total NSC activity. The neural stem cells gave rise to neurospheres containing neurons, astrocytes and oligodendrocytes.