DOG patrol

A helicase protein, named DOG-1, is important for maintaining the stability of guanine-rich tracts of DNA.

Jonathan Weitzman(
Jul 14, 2002

The importance of maintaining genome integrity is highlighted by diseases that arise upon loss of the mechanisms that ensure correct DNA replication and repair. In an Advanced Online Publication in Nature Genetics, Iris Cheung and colleagues describe a mutator phenotype in Caenorhabditis elegans that is associated with deletions in polyguanine tracts and is caused by disruption of a new gene that they have nicknamed dog-1 (for deletions of guanine-rich DNA) (Nat Genet 2002, DOI:10.1038/ng928).

The dog-1 gene encodes a protein containing a DEAH helicase domain. Disruptions within the dog-1 gene, or RNAi experiments, led to variable deletions of G-rich tracts around the C. elegans genome, without affecting other repeat structures or telomeres. Deletions were generally observed in tracts containing more than 22 guanine nucleotides.

Cheung et al. propose that DOG-1 is important for resolving secondary structures of G-rich DNA and predict that homologs may...

Interested in reading more?

Become a Member of

Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!
Already a member?