Green flies
In the December 18 Proceedings of the National Academy of Sciences, Xavier Morin and colleagues describe a gene-trap strategy that generates green fluorescent protein (GFP) fusions and allows the study of protein distribution and subcellular localization in living flies (Proc Natl Acad Sci USA 2001, 98:15050-15055).They created a protein-trap transposon (PTT), a P element containing an artificial exon encoding GFP and flanked by splice acceptor and donor sequences. They derived over 600 fluoresc
Dec 18, 2001
In the December 18
They created a protein-trap transposon (PTT), a P element containing an artificial exon encoding GFP and flanked by splice acceptor and donor sequences. They derived over 600 fluorescent
Characterization of several of these revealed that in most cases splicing occurred correctly and fusions recapitulated endogenous expression of the trapped gene. Over 40% of characterized lines correspond to genes that were not predicted by the