The TAP-tag (tandem affinity purification) methodology has been effectively used for genome-wide proteomic analysis in yeast. Applications in higher eukaryotes have been hindered by the technical difficulties due to expression of endogenous untagged proteins. In an Advanced Online Publication in
This approach was validated by isolating components of the exosome — a multienzyme complex of exoribonucleases, from
The iTAP approach will facilitate functional proteomic projects in higher eukaryotes.