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Mitochondrial DNA homoplasmy

Somatic point mutations in mitochondrial DNA (mtDNA) have been linked to aging and cancer. Each cell contains a large number of mtDNA molecules, so any mutation requires a process of clonal expansion in order to reach homoplasmy (close to 100%) and to exert a phenotypic influence. In the April 16 Proceedings of the National Academy of Sciences, Ekaterina Nekhaeva and colleagues document the frequency of mtDNA mutations in human tissues (Proc Natl Acad Sci USA 2002, 99:5521-5526).They employed a

Jonathan Weitzman(jonathanweitzman@hotmail.com)

Somatic point mutations in mitochondrial DNA (mtDNA) have been linked to aging and cancer. Each cell contains a large number of mtDNA molecules, so any mutation requires a process of clonal expansion in order to reach homoplasmy (close to 100%) and to exert a phenotypic influence. In the April 16 Proceedings of the National Academy of Sciences, Ekaterina Nekhaeva and colleagues document the frequency of mtDNA mutations in human tissues (Proc Natl Acad Sci USA 2002, 99:5521-5526).

They employed a cell-by-cell sequence-specific method to study buccal epithelial cells and cardiomycoytes from aged individuals (up to 109 years old!). They present evidence for intercellular clonal expansion of mtDNA mutations in cells from aged tissues; they found that the rate of spread to homoplasmy was very rapid. The spectra of mtDNA mutations were different in the two cell types and probably reflect a difference in the mechanisms of...

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