Different skeletal myofibre types are known to express distinct muscle myosin isoforms. In the Early Edition of the
They used high-density oligonucleotide arrays (from Affymetrix) to measure gene expression profiles in the extraocular muscle (EOM), jaw and hindlimb muscles of mice. A third of the genes examined were expressed in each muscle type. Profile analysis indicated that EOM is a distinct muscle allotype defined by the expression of 400 EOM-specific genes. These include genes implicated in sarcomeric organisation and excitation-contraction coupling.
Thus, microarrays can be used to define the molecular basis for myofibre classes and to characterize genes contributing to skeletal muscle biology.