For the first time, scientists have described in this week's
"Nobody had ever dreamt you could phosphorylate with a donor other than ATP," said senior author Solomon Snyder at Johns Hopkins University in Baltimore, who, along with colleagues, suggested a decade ago that inositol pyrophosphates such as diphosphoinositol pentakisphosphate (IP7) might serve as phosphorylating agents due to their highly energetic pyrophosphate bonds.
"ATP phosphorylation has heretofore been regarded as the primary mode of all cellular signaling in biology," Snyder told
In the current study, the group synthesized IP7 and radiolabeled the putative donor pyrophosphate. As a control to ensure any apparent...