Re-evaluating the yeast genome
Finding genes within genomic sequence remains a major challenge, even for a genome that has undergone extensive annotation such as that of the yeast Saccharomyces cerevisiae. In January Nature Biotechnology, Anuj Kumar and colleagues at Yale University describe an integrated genomics approach for gene hunting (Nat Biotechnol 2002, 20:58-63).Kumar et al. performed a large-scale insertional mutagenesis screen using a modified transposon as a LacZ-reporter gene-trap. Candidate genes were then verif
Jan 2, 2002
Finding genes within genomic sequence remains a major challenge, even for a genome that has undergone extensive annotation such as that of the yeast
Kumar
This approach led them to identify 137 previously missed genes, many of which encode for short polypeptides or are antisense to annotated genes. Both of these classes of genes are often missed by traditional gene-hunting procedures.