Silencing in mice

An efficient protocol has been described for RNA interference silencing in postnatal mice

Jonathan Weitzman(

In an Advanced Online Publication in Nature Genetics, David Lewis and colleagues describe a simple technique for silencing gene expression in postnatal mice using RNA interference (RNAi) (Nat Genet 29 July 2002, doi:10.1038/ng944).

The authors exploited a 'high-pressure delivery' technique to deliver siRNA (short interfering RNA) to the organs of postnatal mice. They injected plasmid solutions into the tail vein and monitored expression of co-injected constructs encoding a firefly luciferase reporter gene. They achieved up to 90% inhibition levels in the liver, spleen, kidney, lung and pancreas. Inhibition of gene expression in the liver was dose-dependent — as little as 0.05μg siRNA caused a 36% reduction in luciferase gene expression. Lewis et al. also show that injecting siRNA could effectively inhibit transgene expression.

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