Systematic proteomics in yeast
Large-scale purification and mass spectrometry has been used to characterize hundreds of multiprotein complexes in yeast.
Jan 9, 2002
In January 10
Analysis of 1,739 tagged genes lead them to the identification of 98 known nonredundant multiprotein complexes and 134 new complexes. They found partners for 78% of proteins and isolated complexes containing from 2 to 83 components (with an average of 12 per complex). Using a similar approach, Yuen Ho and researchers at the Canadian company MDS Proteomics began with 725 carefully chosen yeast bait proteins tagged with a Flag epitope (