Protein nitration at tyrosine is associated with dozens of pathologies, including transplant rejection, cancer and Parkinson's disease. In the October 9
They used a well-characterized monoclonal antibody recognizing nitrotyrosine to detect nitrated proteins in cytokine-stimulated human cells. Protein extracts were separated using two-dimensional gel electrophoresis and the identity of immunoreactive proteins was determined by mass spectroscopy. Manganese superoxide dismutase (MnSOD) was the predominant immunoreactive nitrated protein.
This study presents a systematic experimental approach to...