Transcriptional switch
Ordered acetylation of the HMGI(Y) protein controls enhanceosome stability and switches on gene expression.
Aug 14, 2001
Transcription is regulated by the coordinated assembly of protein complexes that synergize to switch on gene expression. The virally induced enhanceosome of the interferon-β (IFN-β) gene is one of the best characterized transcriptional switches. In the August 10
The HMGI(Y) protein is a target for acetylation by two different enzymes (CBP and PCAF/GCN5) on distinct lysine residues (Lys65 and Lys71, respectively). Munshi
They performed chromatin immunoprecipitation experiments to demonstrate that K71 acetylation coincides with enhanceosome assembly and activation (3 hours-post infection), whereas Lys65 acetylation occurred at the time of enhanceosome disruption (after 6 hours). Also, Lys71...